Glycans from streptococcal cell walls. The molecular structure of an antigenic diheteroglycan of glucose and galactose from Streptococcus faecalis.

نویسندگان

  • J H Pazur
  • A Cepure
  • J A Kane
  • C G Hellerquist
چکیده

A complete molecular structure for an antigenic glycan isolated from the cell wall of Streptococcus faecalis is being proposed on the basis of analytical data obtained from methylation, acetolysis, and periodate oxidation experiments and on the basis of the nature of the oligosaccharide fragments isolated from partial acid hydrolysates of the glycan. The antigen is a diheteroglycan composed of a main chain of trisaccharide units, glucose-P(1,6)-glucose-p (1,4)-galactose, joined by p(1,4) linkages with lactosyl and cellobiosyl side chains attached by p(1,4) linkages at alternate glucose residues of the main chain. The proposed structure for the glycan is consistent with the immunochemical properties of the polymer. Quantitative precipitin inhibition studies clearly indicate that lactosyl residues are the immunodominant determinants of the glycan.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Glycans from Streptococcal Cell Walls

The cell wall of Streptococcus faecalis, strain N, has been found to contain two antigenic glycans, a diheteroglycan of glucose and galactose and a tetraheteroglycan of rhamnose, glucose, galactose, and N-acetylgalactosamine. The present report describes the isolation, purification, and structural and immunochemical characterization of the hitherto unknown diheteroglycan. A tentative structure ...

متن کامل

Filamentous capsulated streptococci from the human respiratory tract. I. Antigenic attributes of provisional capsular type 83 and its relationship to streptococci of so-called group M.

Two immunologically reactive polysaccharides have been isolated from the cell walls and from culture filtrates of a filamentous alpha-hemolytic streptococcus provisionally designated capsular type 83. Both polysaccharides were purified by diethylaminoethyl-cellulose chromatography. Analysis indicates that the capsular polysaccharide consists of galactose and phosphorus, whereas the cell wall po...

متن کامل

beta-D-Glucose 1-phosphate. A structural unit and an immunological determinant of a glycan from streptococcal cell walls.

Glycose 1-phosphate moieties are emerging as important structural units of macromolecular substances imparting special biological functions to these molecules. In the present study, beta-D-glucose 1-phosphate moieties are shown to be structural units and immunological determinants of a bacterial glycan. The glycan is a tetraheteroglycan from the cell wall of Streptococcus faecalis, strain N and...

متن کامل

The Cell Walls of Group D Streptococci

Group D Types 1 and 26 cell walls and the corresponding type-specific carbohydrates, extracted from the walls by various means, contain rhamnose, glucose, galactose, N-acetylglucosamine, and N-acetylgalactosamine. Mucopeptide elements are also present in the walls and in enzymatically-extracted carbohydrates. Types 1 and 26 carbohydrates extracted by formamide contain no mucopeptide, but the se...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Journal of biological chemistry

دوره 248 1  شماره 

صفحات  -

تاریخ انتشار 1973